Show simple item record Fritts, Ryan K. LaSarre, Breah Stoner, Ari M. Posto, Amanda L. McKinlay, James B. 2018-07-05T16:59:38Z 2018-07-05T16:59:38Z 2017-02
dc.identifier.citation Fritts, RK, B LaSarre, AM Stoner, AL Posto, and JB McKinlay. 2017. A Rhizobiales-specific unipolar polysaccharide adhesin contributes to Rhodopseudomonas palustris biofilm formation across diverse photoheterotrophic conditions. Applied and Environmental Microbiology, 83. en
dc.description Post-print, accepted manuscript version en
dc.description.abstract Bacteria predominantly exist as members of surfaced-attached communities known as biofilms. Many bacterial species initiate biofilms and adhere to each other using cell surface adhesins. This is the case for numerous ecologically diverse Alphaprotebacteria, which use polar exopolysaccharide adhesins for cell-cell adhesion and surface attachment. Here, we show that Rhodopseudomonas palustris, a metabolically versatile member of the alphaproteobacterial order Rhizobiales, contains a functional unipolar polysaccharide (UPP) biosynthesis gene cluster. Deletion of genes predicted to be critical for UPP biosynthesis and export abolished UPP production. We also found that R. palustris uses UPP to mediate biofilm formation across diverse photoheterotrophic growth conditions, wherein light and organic substrates are used to support growth. However, UPP was less important for biofilm formation during photoautotrophy, where light and CO2 support growth, and during aerobic respiration with organic compounds. Expanding our analysis beyond R. palustris, we examined the phylogenetic distribution and genomic organization of UPP gene clusters among Rhizobiales species that inhabit diverse niches. Our analysis suggests that UPP is a conserved ancestral trait of the Rhizobiales but that it has been independently lost multiple times during the evolution of this clade, twice coinciding with adaptation to intracellular lifestyles within animal hosts. en
dc.language.iso en en
dc.publisher Applied and Environmental Microbiology en
dc.relation.isversionof en
dc.subject Rhodopseudomonas en
dc.subject adhesin en
dc.subject biofilm en
dc.subject holdfast en
dc.subject phylogenetic analysis en
dc.subject unipolar polysaccharide en
dc.title A Rhizobiales-Specific Unipolar Polysaccharide Adhesin Contributes to Rhodopseudomonas palustris Biofilm Formation across Diverse Photoheterotrophic Conditions en
dc.type Article en
dc.identifier.doi 10.1128/AEM.03035-16

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