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dc.contributor.author Fornelli, Luca
dc.contributor.author Durbin, Kenneth R
dc.contributor.author Fellers, Ryan T
dc.contributor.author Early, Bryan P
dc.contributor.author Greer, Joseph B
dc.contributor.author LeDuc, Richard D
dc.contributor.author Compton, Phillip D
dc.contributor.author Kelleher, Neil L
dc.date.accessioned 2016-07-18T18:03:32Z
dc.date.available 2016-07-18T18:03:32Z
dc.date.issued 2016-08
dc.identifier.citation Forthcoming en
dc.identifier.uri http://hdl.handle.net/2022/20923
dc.description.abstract Over the past decade, developments in high resolution mass spectrometry have enabled the high throughput analysis of intact proteins from complex proteomes, leading to the identification of thousands of proteoforms. Traditionally, top-down proteomics experiments relied on hybrid ion trap – Fourier transform mass spectrometers combined with data-dependent acquisition strategies. Here, we used a benchtop quadrupole – Orbitrap instrument coupled with unique data acquisition software to identify and characterize nearly 2,000 proteoforms at 1% false discovery rate from human fibroblasts. en
dc.description.sponsorship National Institute of General Medical Sciences of the National Institutes of Health. L.F. would like to acknowledge the Swiss National Science Foundation for support of an Early Postdoc Mobility fellowship en
dc.language.iso en_US en
dc.publisher Forthcoming en
dc.relation.isversionof Forthcoming en
dc.rights Creative Commons Attribution 4.0 license (http://creativecommons.org/licenses/by/4.0/) en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Top-down proteomics; gas phase fractionation; Orbitrap; quadrupole; data-dependent acquisition; false-discovery rate; mass spectrometry; proteoform; AUTOPILOT; medium/high en
dc.title Advancing top-down analysis of the human proteome using a benchtop quadrupole-Orbitrap mass spectrometer en
dc.type Dataset en
dc.altmetrics.display true en


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