Browsing Lab Protocols by Issue Date

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  • Dustin Reichard, Nicole Gerlach (2013-03-29)
    Directions for making subcutaneous hormone implants (crystalline testosterone in Silastic tubing).
  • Jodie Jawor, Danielle Whittaker, Nicole Gerlach, Sarah Wanamaker (2013-03-29)
    Standard Operating Procedure for storing and using regulated substances (steroid hormones).
  • Nicole Gerlach, Kimberly Rosvall (2013-03-29)
    Protocols for administering subcutaneous silastic hormone implants. Includes descriptions of the surgical method, including protocols for using inhaled anesthetics, and the non-surgical trochar implantation method.
  • Kimberly Rosvall, Dustin Reichard (2013-03-29)
    Protocol for corticosterone EIA, based on the CORT kit from Caymen
  • Nicole Gerlach (2013-03-29)
    Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs. Similar to the full protocol, but modified so that radiolabeled testosterone is only added to the standards, not the samples.
  • Danielle Whittaker (2013-03-29)
    Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs
  • Joe Casto, Nicole Gerlach, Ketterson/Nolan research group (2013-03-29)
    A spreadsheet for calculating testosterone concentrations based on enzyme immunoassay results. Has spaces for the radiation counts per minute to be entered to calculate recovery rates, the concentration from the plate ...
  • Nicole Gerlach (2013-03-29)
    Recipe for mixing a solution of adrenocorticotropic hormone (ACTH), and protocol for giving ACTH injections as part of an ACTH challenge
  • Joe Casto, Lynn Siefferman, Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of gonadotropin releasing hormone (c-GnRH) from a powder form.
  • Joe Casto, Joel McGlothlin, Ellen Ketterson (2013-03-29)
    Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.
  • Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of lutenizing hormone (LH) from a purchased powder.
  • Jen Grindstaff (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of enzyme-linked immunosorbent assays (ELISAs).
  • Jen Grindstaff (2013-03-29)
    Protocol for an enzyme-linked immunosorbent assay (ELISA) which gives a quantitative measure of total IgG levels in plasma from Japanese quail.
  • Joe Casto, Nicole Gerlach (2013-03-29)
    Protocol for administering a PHA immune challenge to nestlings and adults.
  • Devin Zysling (2013-03-29)
    Protocol for measuring immune response to injection of sheep red blood cells (SRBC)
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
  • Ketterson/Nolan research group (2013-03-29)
    Protocol for collecting blood samples for later DNA extraction
  • Danielle Whittaker, Nicole Gerlach (2013-03-29)
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...
  • Danielle Whittaker (2013-03-29)
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.

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