Browsing Lab Protocols by Issue Date

Sort by: Order: Results:

  • Ian Parker-Renga (2013-03-29)
    An ethogram describing various categories of observed behavior, specific for female juncos.
  • Jen Grindstaff (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of enzyme-linked immunosorbent assays (ELISAs).
  • Joe Lipar (2013-03-29)
    This protocol can be used to measure the concentration of steroid hormones in yolk samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington and Dr. ...
  • Danielle Whittaker (2013-03-29)
    Questions about methodology for collecting preen oil samples from dark-eyed juncos.
  • Unsure original creator, possibly updated by Kristal Cain? (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of laboratory tests (ELIZA, Hormone assay, Carotenoid HPLC)
  • Nicole Gerlach (2013-03-29)
    Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs. Similar to the full protocol, but modified so that radiolabeled testosterone is only added to the standards, not the samples.
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for extraction of RNA from avian tissue using Trisol and includes instructions for gel electrophoresis.
  • Ketterson/Nolan research group, Sara Schrock (2013-03-29)
    Recipe for making Longmire's solution, a cell lysis buffer for storing blood samples for DNA analysis. Also includes recipes for stock solutions used in making Longmire's solution.
  • From Peter Dunn's lab; unsure if updated by Kettlab members (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the salt solution extraction buffer.
  • Ketterson/Nolan research group (2013-03-29)
    Protocol for collecting blood samples for later DNA extraction
  • Joe Casto, Lynn Siefferman, Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of gonadotropin releasing hormone (c-GnRH) from a powder form.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
  • Danielle Whittaker, Nicole Gerlach (2013-03-29)
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...
  • Danielle Whittaker (2013-03-29)
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
  • Danielle Whittaker (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
  • Joe Casto, Joel McGlothlin, Ellen Ketterson (2013-03-29)
    Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for reverse transcriptase / cDNA synthesis from extracted RNA samples.
  • Jen Grindstaff (2013-03-29)
    Protocol for an enzyme-linked immunosorbent assay (ELISA) which gives a quantitative measure of total IgG levels in plasma from Japanese quail.
  • Joe Casto, Nicole Gerlach (2013-03-29)
    Protocol for administering a PHA immune challenge to nestlings and adults.
  • Christine Bergeon Burns (2013-03-29)
    Recipe for mixing a solution of lutenizing hormone (LH) from a purchased powder.

Search IUScholarWorks


Advanced Search

Browse

My Account