Lab Protocols

 

Disclaimer
These protocols are presented so that others may adapt and possibly improve upon them. Every effort was made to make them accurate, but in some cases they were a work in progress and should not be relied on without independent validation.

Sensitive Issues

We recognize and acknowledge that certain audiences may be offended by research on animals and note that throughout the research we have taken all possible measures to preserve the welfare of the birds and cause no undue harm or interference. We conduct our research under the oversight of a federally mandated animal care committee, and we hope that our findings will ultimately be helpful to the animals themselves by learning more about their natural history and how best to contribute to their conservation.

Licensing and Rights
Attribution-NonCommercial-ShareAlike
CC BY-NC-SA: http://creativecommons.org/licenses/by-nc-sa/3.0/

Recent Submissions

  • Joe Casto, Jodie Jawor, Danielle Whittaker (2013-03-29)
    This protocol can be used to measure the concentration of steroid hormones in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. ...
  • Unsure original creator, possibly updated by Kristal Cain? (2013-03-29)
    Protocol for cleaning radio-labeled steroid hormone
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for extraction of RNA from avian tissue using Trisol and includes instructions for gel electrophoresis.
  • Ketterson/Nolan research group, Sara Schrock (2013-03-29)
    Recipe for making Longmire's solution, a cell lysis buffer for storing blood samples for DNA analysis. Also includes recipes for stock solutions used in making Longmire's solution.
  • Joe Casto, Jodie Jawor (2013-03-29)
    This protocol can be used to measure the concentration of corticosterone in small plasma samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington. ...
  • Lynn Siefferman (2013-03-29)
    Protocol for using the spectrometer to analyze color spectrum from feather samples.
  • George Gee, Elaina Tuttle, Tracey Kast (2013-03-29)
    Protocol for collecting sperm from songbirds, as well as for counting sperm samples. Also includes data sheet.
  • From Peter Dunn's lab; unsure if updated by Kettlab members (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the salt solution extraction buffer.
  • Danielle Whittaker (2013-03-29)
    Questions about methodology for collecting preen oil samples from dark-eyed juncos.
  • Ian Parker-Renga (2013-03-29)
    An ethogram describing various categories of observed behavior, specific for female juncos.
  • Joe Lipar (2013-03-29)
    This protocol can be used to measure the concentration of steroid hormones in yolk samples. This protocol has been copied and modified from protocols provided by Dr. John Wingfield at the University of Washington and Dr. ...
  • Unsure original creator, possibly updated by Kristal Cain? (2013-03-29)
    Protocol to prepare frozen egg yolks for use in a variety of laboratory tests (ELIZA, Hormone assay, Carotenoid HPLC)
  • Steve Schoech (2013-03-29)
    A spreadsheet for calculating steroid hormone concentrations from radioimmunoassay results. Calculates concentrations of progesterone, testosterone, estradiol, corticosterone, and dihydrotestosterone.
  • Christine Bergeon Burns, Kimberly Rosvall (2013-03-29)
    Protocol for quantitative PCR to analyse RNA concentration from cDNA.
  • Ketterson/Nolan research group (2013-03-29)
    Protocol for collecting blood samples for later DNA extraction
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses IBI Scientific MINI Genomic DNA extraction kits. Includes checklist for each step.
  • Danielle Whittaker, Nicole Gerlach (2013-03-29)
    Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...
  • Danielle Whittaker (2013-03-29)
    Protocol for PCR of extracted avian DNA using primers that can determine the sex of the samples. Uses agarose gels to determine the amplified alleles.
  • Danielle Whittaker (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction.
  • Nicole Gerlach (2013-03-29)
    Protocol for extracting DNA from whole blood stored in lysis buffer. Uses the phenol-chloroform method of extraction. Modified from full protocol to include checklist for each step.

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